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Understanding melting temperature (Tm)
The DNA primer melting temperature (Tm) - YouTube
FastPCR manual
How to calculate melting temperature of DNA "2+4 rule of thumb" - YouTube
SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule" This equation is: Tm = 2(A+T) + 4(G+C) Where A = # of